Impact of inactivated extracellular proteases on the modified flagellin type III secretion pathway of Bacillus halodurans.
نویسندگان
چکیده
The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (Deltahag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced through gene-targeted inactivation of extracellular proteases.
منابع مشابه
Inactivation of extracellular proteases in Bacillus halodurans and the impact 2 on its modified flagellin type III secretion pathway 3 4
9 The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (∆hag) was modified by 10 inactivation of fliD. An in frame flagellin fusion polypeptide construct was expressed and the 11 heterologous peptides secreted as flagellin fusion monomers. The stability of the secreted 12 monomers was significantly enhanced through gene-targeted inactivation of extracellular 13 proteases. 14 C...
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 75 1 شماره
صفحات -
تاریخ انتشار 2009